百子莲胚性愈伤组织在超低温过程中的蛋白质组学研究
文章编号：2095-1191（2019）11-2410-11
Proteomics analysis of embryogenic callus of Agapanthus praecox during cryopreservation
LYU Shan， ZHANG Di*
（School of Design， Shanghai Jiaotong University， Shanghai 200240， China）
Abstract：【Objective】The aim of the study was to reveal the response and protection mechanism of plant embryogenic cells under complex stress during cryopreservation on the proteomics level， and to find the differentially expressed proteins（DEPs） during cryopreservation of embryogenic callus of Agapanthus praecox to eich the molecular biology theory. 【Method】iTRAQ（isobaric tags for relative and absolute quantification） technology was performed to identify DEPs in the four key steps of cryopreservation（preculture，vitrification dehydration，dilution，recovery） of the embryogenic callus of A. praecox，bioinformatics analysis method was used to construct the protein regulatory network in response to complex stress during cryopreservation and select stressed-protected proteins in plants. 【Result】A total of 2730 proteins were identified，of which 821 DEPs were selected. GO annotation analysis showed that these DEPs were mainly involved in biological process such as metabolic process，cellular process and responses to stimulus，while catalytic activity and molecular binding were the most important parts of molecular function. KEGG pathway analysis indicated that DEPs were mainly eiched in energy metabolism，protein synthesis and degradation，signal transduction and membrane repair. The DEPs interaction regulatory network was constructed by STRING software，it was found that up-regulated DEPs were mainly involved in carbohydrate metabolism，oxidation-reductive process and ribosome translation，while down-regulated DEPs were mainly involved in ROS metabolism，DNA repair，inhibition of programmed cell death（PCD） process and cytoskeleton. In addition，96 DEPs related to stress protection were detected for energy metabolism，redox homeostasis，protein synthesis and degradation and signal transduction. 【Conclusion】Energy metabolism，oxidative stress，protein degradation and PCD events of embryogenic callus of A. praecox increase during cryopreservation;while the ROS clearance and stress defense process of cells decrease greatly. Embryogenic callus of A. praecox form resistance protection mechanism with glycometabolism and antioxidant system as the majority under cryopreservation compound stress.